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Wolverine stack calculator
"Wolverine" is BPC-157 and TB-500 run together, sold either as one pre-blended vial or bought as two separate vials. The arithmetic differs between the two. In a blend, one draw carries both peptides in a fixed ratio, so you set the dose of one and the other comes along with it. With separate vials, each has its own concentration and its own draw. This calculator does both. For why the two are paired and what the evidence does and does not show, read the BPC-157 + TB-500 stack guide.
Blend arithmetic
In a blended vial both peptides dissolve in the same water, so each has its own concentration: mg of that peptide ÷ mL of water. You pick a dose for one of them and the calculator turns it into a volume. That same volume carries the other peptide in whatever proportion the vial was packed. In a 5 mg + 5 mg vial the ratio is 1:1, so 250 mcg of BPC-157 always arrives with 250 mcg of TB-500. On a U-100 insulin syringe, 100 units is 1 mL, so units = (dose ÷ concentration) × 100.
| Blend + water | Each peptide | 250 mcg of each | 500 mcg of each | Doses at 500 mcg |
|---|---|---|---|---|
| 5 + 5 mg in 2 mL | 2.5 mg/mL | 10 u | 20 u | 10 |
| 5 + 5 mg in 3 mL | 1.67 mg/mL | 15 u | 30 u | 10 |
| 10 + 10 mg in 2 mL | 5 mg/mL | 5 u | 10 u | 20 |
| 10 + 10 mg in 3 mL | 3.33 mg/mL | 7.5 u | 15 u | 20 |
| 10 + 10 mg in 4 mL | 2.5 mg/mL | 10 u | 20 u | 20 |
Red = a draw small enough that a one-unit misread is a 20% dose error. More water moves the same dose further up the barrel. The water never changes how much peptide is in the vial, only how many units each dose takes.
Blend or separate vials?
The blend is convenient: one vial, one draw, one injection. The cost is that it fixes two things you might otherwise want to set independently. The ratio is whatever the vial was packed at, so changing the amount of one peptide changes the other by the same proportion. The schedule is shared, so both go in on the same days. Write-ups of the two peptides on their own often give BPC-157 daily and TB-500 as a weekly amount over fewer injections. A fixed blend cannot follow both patterns at once. If your prescriber's plan has different amounts or different days for each, separate vials are the way to do it. The calculator's second mode handles the two draws. The peptide blend calculator covers three- and four-peptide mixes such as GLOW and KLOW.
Storage and the vial's clock
Reconstituted peptide is usually kept refrigerated at 2–8 °C and used within about 28 days, the working figure in the peptide storage guide. Check "Vial lasts" above against that window. A 10 mg + 10 mg vial at 250 mcg of each per day holds 40 doses, longer than the window, so roughly the last 12 days' worth would be past it. Use bacteriostatic water for a vial you will puncture daily and label it with the concentration and the date. Do not use it if it turns cloudy or has particles in it. The BPC-157 reconstitution guide walks through the mixing steps, which are the same for a blend.
BPC-157 and TB-500 are not approved medicines anywhere we know of. They are sold as research chemicals with no guarantee that a vial holds what its label says, or that it is sterile. A blend adds a second unverified amount to the same vial. The evidence for each is mostly animal work and no controlled human trial has tested the combination (the stack guide goes through it). The FDA's list of bulk substances that may present significant safety risks in compounding, updated April 2026, names both, citing the risk of immune reactions and peptide impurities. For where compounding stands, see US peptide status. Both are prohibited in sport under the WADA list, so if you are tested, do not use them. This calculator does arithmetic on amounts you enter; it is not medical advice and not a recommendation to use either peptide.
One stack, one timeline
OptiPin logs a blend as one stack, tracks what is left in each vial, rotates injection sites and warns you before a vial runs out or passes its date. Pain or range-of-motion scores sit on the same timeline, so you can see whether anything changed. iOS, on-device, no account.
Get OptiPinFrequently asked questions
What is the Wolverine stack?
It is the name sellers and forums give to BPC-157 and TB-500 run together, either as one pre-blended vial or as two separate vials. The common blended vials are 5 mg + 5 mg (10 mg total) and 10 mg + 10 mg (20 mg total). Neither peptide is an approved medicine and no controlled human trial has tested the combination.
How much water do I add to a 10 mg Wolverine blend?
The water does not change the dose, only how many units it takes. A 5 mg + 5 mg vial with 2 mL is 2.5 mg/mL of each peptide, so 250 mcg of BPC-157 is 10 units and brings 250 mcg of TB-500 with it. With 3 mL it is 15 units. Aim for draws of roughly 10 to 30 units, where a one-unit misread is a small share of the dose.
Can I dose BPC-157 and TB-500 separately from a blended vial?
No. A blend fixes the ratio and the schedule: every unit you draw carries both peptides in the proportion they were packed, on the same days. If you want different amounts of each, or TB-500 on fewer days than BPC-157, that needs two separate vials. The calculator's separate-vials mode handles that case.
How many doses are in a Wolverine vial?
Divide the amount of either peptide by the amount per draw. A 5 mg + 5 mg vial drawn at 500 mcg of each holds 10 doses, which is 10 days at one injection a day. A 10 mg + 10 mg vial at the same draw holds 20. Reconstituted peptide is usually kept for about 28 days refrigerated, so a 20-dose vial used daily fits that window.
Does this calculator tell me what dose to take?
No. It does the arithmetic for amounts you enter. BPC-157 and TB-500 are unapproved, investigational compounds sold for research use with no guarantee of strength or purity. Both are prohibited in tested sport. Any decision about using them belongs with a clinician.
Sources
- Certain Bulk Drug Substances for Use in Compounding that May Present Significant Safety Risks, US Food and Drug Administration, content current as of 04/22/2026. Entries for BPC-157 and Thymosin Beta-4 Fragment (LKKTETQ).
- World Anti-Doping Code: The Prohibited List, World Anti-Doping Agency, 2026 list. BPC-157 is named under S0 (non-approved substances) and "Thymosin-ß4 and its derivatives e.g. TB-500" under S2.
Blend sizes are the ones commonly listed by sellers in 2026. Research-grade vials vary, so always calculate from the amounts printed on your own vial. U-100 syringe: 100 units = 1 mL.
Related
BPC-157 + TB-500 stack guide · BPC-157 reconstitution · GLOW and KLOW blend calculator · BPC-157 tracker · TB-500 tracker · Peptide calculator · All calculators